Expression, Purification, Characterization and Protein Catalysis of mutated Fluorescent Proteins

Expression, Purification, Characterization and Protein Catalysis of mutated Fluorescent Proteins

Internship Description

A fluorescent  protein variant was tailor-made as a suitable host for the incorporation of artificial metal centers through in silico removal of metal binding motifs and improvement of thermal, salt and solvent stability. Through introduction of mutations, metalloproteins are generated, which posses reactivities nature does not provide. These metalloproteins will be tested in aqueous catalysis reactions and the influence of mutations on selectivity and  reactivity will be studied.​​

Deliverables/Expectations

Students shall extend  their general knowledge and skills in molecular  biology and protein  biochemistry. An emphasis will be put on expression, purification and characterization techniques. If capable mutant  proteins will be  crystallized  to determine structural properties. Students will be taught to work independently on projects, yet strengthening their critical sense to develop new ideas. In the course of the internship students shall demonstrate this understanding during oral resentations  and one  final written report.​

Faculty Name

Magnus Rueping

Field of Study

​Chemistry/Biochemistry/Molecular Biology