Expression, Purification, Characterization of Proteins for Biocatalysis

Expression, Purification, Characterization of Proteins for Biocatalysis

Internship Description

A fluorescent protein variant was tailor-made as a suitable host for the incorporation of artificial metal centers through in silico removal of metal binding motifs and improvement of thermal, salt and solvent stability. These new metalloproteins possess reactivities nature does not provide. The further goal will be the examination of the new metalloprotein in aqueous catalysis reactions and the examination of the influence of further mutations on selectivity and reactivity.
Project-duration will be 3-6 month, details of arrival/departure dates to be discussed.

Deliverables/Expectations

​Students shall extend their general knowledge and skills in molecular biology and protein biochemistry. An emphasis will be put on expression, purification and characterization techniques. If capable mutant proteins will be crystallized to determine structural properties. Students will be taught to work independently on projects, yet strengthening their critical sense to develop new ideas. In the course of the internship students shall demonstrate this understanding during oral presentations and one final written report.

Faculty Name

Magnus Rueping

Field of Study

​Chemistry/Biochemistry/Molecular Biology