Spatio temporal analysis of expression of genes controlling assymetric stem cell division and tissue patterning in plants

Spatio temporal analysis of expression of genes controlling assymetric stem cell division and tissue patterning in plants

Internship Description

BIRDs nuclear factors have been described to regulate root growth through association with the transcription factors SCARECROW and SHORTROOT, however their function in other organs remain to be elucidated. Here we propose to dissect network function in lateral roots and leaves. We will determine their physical associations spatially and during different developmental stages. We will also assess whether their target are regulated similarly.

Objectives: In this project we aim to dissect how BIRD proteins regulate leave tissue patterning and map their localization in different mutant backgrounds. In addition, we will dissect binding sites in different target genes and alter specific binding by site directed mutagenesis.

 

Technologies: confocal imaging microscopy, site directed mutagenesis, promoter activities using dual luciferase, plant phenotyping, cloning using gateway technology

 

Deliverables/Expectations

Map the expression of the genes at different developmental stages and dissect thein binding motifs

Faculty Name

Ikram Blilou

Field of Study

Plant Biology