Microfluidics-based single-molecule fluorescence imaging of nanoscopic cellular interactions


Project Description

The adhesion of cells to the endothelium occurs through spatio-temporally regulated interactions that are mediated by multiple intra- and inter-cellular components. The mechanism of cell adhesion has been investigated primarily using ensemble-based experiments, which provides indirect information about how individual molecules work in such a complex system. In this project, we develop microfluidics-based in-vitro live-cell single-molecule fluorescence imaging technique to unravel nanoscopic spatiotemporal interaction between adhesion molecules during the cell migration. Specifically, we aim to address some key questions in the initial step of hematopoietic stem cell homing mediated by selectin-ligand interactions.
Program - BioScience
Division - Biological and Environmental Sciences and Engineering
Field of Study - ​Fluorescence microscopy, Micro/nano fabrication, Optics, Biophysics, Immunology

About the

Satoshi Habuchi

Professor, Bioscience<br/>Chair, Bioscience Program

Satoshi Habuchi

Professor Habuchi's research focuses on the development of tools and materials for fluorescence molecular imaging. His research interests include the development of microfluidics-based super-resolution/single-molecule fluorescence imaging platform for studying cellular interactions in the presence of external force, development of near-infrared/short wavelength infrared fluorescent nanoparticles using conjugated polymers for single-molecule/particle fluorescence imaging in biological tissues, and development of new single-molecule tracking methods to capture hidden nonrandom motion and conformational dynamics at the molecular level.

Desired Project Deliverables

​Development of new microfluidics-based live-cell single-molecule fluorescence imaging technique.Characterization of nanoscopic spatiotemporal interaction between selectins and their ligands occurring during the cell migration.​