A peculiarity of human reproduction is the high rate of developmental failure before and after the time of embryo implantation. Understanding why early pregnancy failures occur is a significant question in the field but remains challenging due to the technical and ethical complexities involved in studying these developmental stages. Pluripotent stem cells have recently been used to construct various early developmental models that hold great promise in unlocking the mysteries of early human development and facilitating new reproductive therapies. Human blastoids are one such valuable model for studying early human development. They can be derived from naïve pluripotent stem cells and consist of all three lineages of the preimplantation blastocyst. Current methods using static batch culture to generate human blastoids have limitations, including (i) low yield, (ii) the need for cell aggregation, which complicates genetic or drug screens, (iii) reproducibility issues due to an uncontrolled cellular environment, and (iv) the requirement for Aggrewell or similar confinements that can exert unnatural geometrical strains on blastoids. Additionally, current blastoid models exhibit instabilities in the development of post-implantation lineages. The candidates of this VSRP project are expected to work on developing methods for large-scale production of human blastoids under strict environmental control, improving post-implantation lineage differentiation of blastoids, and characterizing gene expression in a spatially resolved manner in single cells. The successful outcome of the project is expected to significantly advance the development of in vitro human embryo models that faithfully recapitulate specific intricacies of embryogenesis. These models have the potential to be translated into applications in regenerative medicine, disease modeling, and personalized therapies.