An iPSCs-based approach to model Type Two Diabetes in-vitro
ApplyProject Description
Studying the transcriptional and epigenetic mechanisms dysregulated in patients affected by metabolic disorders such as insulin resistance (IR) and type 2 diabetes mellitus (T2DM) is essential to derive efficient pharmacological approaches. We are seeking an outstanding student to work on a project focused on the study of the role of histone modifiers to the onset of metabolic disorders.
Program -
BioScience
Division -
Biological and Environmental Sciences and Engineering
Field of Study -
Molecular and Cellular Biology and/or Bioinformatics
About the
Researcher
Antonio Adamo
Assistant Professor, Bioscience
Professor Adamo's research interests focus on the use of human embryonic stem cells (hESCs) and patient-derived induced pluripotent stem cells (iPSCs) to model the onset and progression of human disorders linked to copy number variations “in a dish.” His team developed the largest cohort of Klinefelter syndrome (karyotype 47,XXY) and high-grade X aneuploid iPSCs (karyotype 48,XXXY and 49,XXXXY) that he uses to study the molecular dysregulations associated with X chromosome aneuploidy during the earliest stages of human development. Professor Adamo’s team combines reprogramming, organoid derivation, and genome editing techniques with a multi-omics approach to identify the transcriptional and epigenetic signatures underlying human diseases.
Desired Project Deliverables
The selected candidate will use human stem cells and terminally differentiated glucose sensitive cell types and will acquire skills in molecular biology techniques including Chromatin Immuno-precipitation (ChIP), quantitative real-time PCR (Q-PCR) and next generation sequencing (NGS).