Development of a novel Stimulated Raman Scattering microscopy system

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Project Description

Microscopy techniques based on vibrational spectroscopy are poised to be part of the next generation of microscopes for biological applications based on their unique chemical contrast and sub-cellular resolution for non-invasive, non-destructive and label free imaging of biological samples as live cells. The project will focus on the development of a fast and low-noise detection system in a setup for microscopic vibrational spectroscopy based on Stimulated Raman Scattering, which is one of the most advanced and sensitive methods for label-free microscopy for bio-imaging. The system will be applied to vibrational imaging of cancer stem cells to unveil their specific bio-chemical signatures. ​ ​​​​
Program - BioScience
Division - Biological and Environmental Sciences and Engineering
Field of Study - ​Electrical Engineering, physics

About the
Researcher

Carlo Liberale

Assistant Professor, Bioscience

Carlo Liberale
Prof. Liberale's research interests are focused on developing and applying label-free chemical imaging techniques based on vibrational spectroscopy (Infrared and Raman micro-spectroscopy) and multi-photon processes (Coherent Raman Microscopy, SHG). One of the main aims of this research activity is to unveil specific bio-chemical signatures of cancer stem cells, with a particular focus on understanding the dysregulation of their lipid metabolism.

He is also interested on using high-resolution 3D printing based on Direct Laser Writing for the fabrication of novel micro-optics, towards the miniaturization of complex optical systems, and of smart micro-/nano-structures to be used as a probe in nanoscale imaging. This research activity takes advantage from an integrated approach that combines design, micro/nanofabrication and optical techniques.​

Desired Project Deliverables

​Learn Coherent Raman Scattering techniques. Design, assemble and test circuitry for multiplexed and low-noise detection in a Stimulated Raman Scattering microscopy setup based on femtosecond broadband laser sources. Demonstrate fast and high S/N ratio imaging with multiplex (broadband) Stimulated Raman Scattering microscopy. ​