Development of a novel Stimulated Raman Scattering microscopy system


Project Description

Microscopy techniques based on vibrational spectroscopy are poised to be part of the next generation of microscopes for biological applications based on their unique chemical contrast and sub-cellular resolution for non-invasive, non-destructive and label free imaging of biological samples as live cells. The project will focus on the development of a fast and low-noise detection system in a setup for microscopic vibrational spectroscopy based on Stimulated Raman Scattering, which is one of the most advanced and sensitive methods for label-free microscopy for bio-imaging. The system will be applied to vibrational imaging of cancer stem cells to unveil their specific bio-chemical signatures. ​ ​​​​
Program - BioScience
Division - Biological and Environmental Sciences and Engineering
Field of Study - ​Electrical Engineering, physics

About the

Carlo Liberale

Assistant Professor, Bioscience

Carlo Liberale
Prof. Liberale's research interests are focused on developing and applying label-free chemical imaging techniques based on vibrational spectroscopy (Infrared and Raman micro-spectroscopy) and multi-photon processes (Coherent Raman Microscopy, SHG). One of the main aims of this research activity is to unveil specific bio-chemical signatures of cancer stem cells, with a particular focus on understanding the dysregulation of their lipid metabolism.

He is also interested on using high-resolution 3D printing based on Direct Laser Writing for the fabrication of novel micro-optics, towards the miniaturization of complex optical systems, and of smart micro-/nano-structures to be used as a probe in nanoscale imaging. This research activity takes advantage from an integrated approach that combines design, micro/nanofabrication and optical techniques.​

Desired Project Deliverables

​Learn Coherent Raman Scattering techniques. Design, assemble and test circuitry for multiplexed and low-noise detection in a Stimulated Raman Scattering microscopy setup based on femtosecond broadband laser sources. Demonstrate fast and high S/N ratio imaging with multiplex (broadband) Stimulated Raman Scattering microscopy. ​