Mapping protein complexes in vivo

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Project Description

During multicellular development, the specification of distinct cell fates is often regulated by the same transcription factors operating differently in distinct cis-regulatory modules. In the model plant Arabidopsis root, distinct cell types are determined by the interacting transcription factors within one contiguous tissue layer. Using fluorescence lifetime microscopy we revealacell type-specific protein complexes that differentially regulate target genes and instruct distinct cell fates. In addition to determining the network function in other organs, we are currently studying how are these complexes established and maintained by generating a temporal interaction map and dissecting the transcriptional regulation by these complexes.We will use the following tools: FRET-FLIM, confocal microscopy imaging, mutants analysis.​
Program - Plant Science
Division - Biological and Environmental Sciences and Engineering
Center Affiliation - Center for Desert Agriculture
Field of Study - ​Developmental biology, cell biology

About the
Researcher

Ikram Blilou

Professor, Plant Science<br/>Chair, Plant Science Program

Ikram Blilou

Blilou's Plant Cell and Developmental Biology research group concentrates on understanding plant developmental processes while focusing on their root system architecture. The date palm, mangrove, and Sodom apple species have shaped their roots to cope with scarce water, high salinity, and poor nutrient availability, characteristics which can be exploited to engineer more resilient crops for arid environments. By revealing strategies employed by the date palm to survive in arid conditions, Blilou's team establishes genetic tools that allow date palms to grow faster, healthier, and with roots that require even less water. LPCDB also studies the regulatory networks that control protein movement and asymmetric cell division in plant roots.

Desired Project Deliverables

​Generate a spatio-temporal protein interaction map in vivo​