Spatio temporal analysis of expression of genes controlling assymetric stem cell division and tissue patterning in plants

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Project Description

BIRDs nuclear factors have been described to regulate root growth through association with the transcription factors SCARECROW and SHORTROOT, however their function in other organs remain to be elucidated. Here we propose to dissect network function in lateral roots and leaves. We will determine their physical associations spatially and during different developmental stages. We will also assess whether their target are regulated similarly.Objectives: In this project we aim to dissect how BIRD proteins regulate leave tissue patterning and map their localization in different mutant backgrounds. In addition, we will dissect binding sites in different target genes and alter specific binding by site directed mutagenesis. Technologies: confocal imaging microscopy, site directed mutagenesis, promoter activities using dual luciferase, plant phenotyping, cloning using gateway technology ​
Program - Plant Science
Division - Biological and Environmental Sciences and Engineering
Center Affiliation - Center for Desert Agriculture
Field of Study - ​Plant Biology​

About the
Researcher

Ikram Blilou

Professor, Plant Science<br/>Chair, Plant Science Program

Ikram Blilou

Blilou's Plant Cell and Developmental Biology research group concentrates on understanding plant developmental processes while focusing on their root system architecture. The date palm, mangrove, and Sodom apple species have shaped their roots to cope with scarce water, high salinity, and poor nutrient availability, characteristics which can be exploited to engineer more resilient crops for arid environments. By revealing strategies employed by the date palm to survive in arid conditions, Blilou's team establishes genetic tools that allow date palms to grow faster, healthier, and with roots that require even less water. LPCDB also studies the regulatory networks that control protein movement and asymmetric cell division in plant roots.

Desired Project Deliverables

​Map the expression of the genes at different developmental stages and dissect thein binding motifs