Spatio temporal analysis of expression of genes controlling assymetric stem cell division and tissue patterning in plants

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Project Description

BIRDs nuclear factors have been described to regulate root growth through association with the transcription factors SCARECROW and SHORTROOT, however their function in other organs remain to be elucidated. Here we propose to dissect network function in lateral roots and leaves. We will determine their physical associations spatially and during different developmental stages. We will also assess whether their target are regulated similarly.Objectives: In this project we aim to dissect how BIRD proteins regulate leave tissue patterning and map their localization in different mutant backgrounds. In addition, we will dissect binding sites in different target genes and alter specific binding by site directed mutagenesis. Technologies: confocal imaging microscopy, site directed mutagenesis, promoter activities using dual luciferase, plant phenotyping, cloning using gateway technology ​
Program - Plant Science
Division - Biological and Environmental Sciences and Engineering
Center Affiliation - Center for Desert Agriculture
Field of Study - ​Plant Biology​

About the
Researcher

Ikram Blilou

Associate Professor, Plant Science<br/>Chair, Plant Science Program

Ikram Blilou

Professor Blilou' s research focuses on how plant cells communicate to transfer positional information and to instruct specific functions during pattern formation. This involves studying regulatory networks that control protein movement and asymmetric cell division in plant roots, by mapping protein complexes in vivo at the cellular resolution, and by unraveling how their distinct spatial distribution leads to specific gene expression and proper cell fate acquisition.

Professor Blilou's research group also aims to understand molecular mechanisms of growth/defense trade-offs in plants by unraveling how the same set of developmental genes can regulate defense response under stress conditions. The team also intends to understand adaptive strategies used by desert plants (using date palms as a model) to survive in hostile conditions.

Desired Project Deliverables

​Map the expression of the genes at different developmental stages and dissect thein binding motifs