An iPSCs-based approach to model Type Two Diabetes in-vitro


Project Description

Studying the transcriptional and epigenetic mechanisms dysregulated in patients affected by metabolic disorders such as insulin resistance (IR) and type 2 diabetes mellitus (T2DM) is essential to derive efficient pharmacological approaches. We are seeking an outstanding student to work on a project focused on the study of the role of histone modifiers to the onset of metabolic disorders.​​​​​​
Program - BioScience
Division - Biological and Environmental Sciences and Engineering
Field of Study - ​Molecular and Cellular Biology and/or Bioinformatics

About the

Antonio Adamo

Assistant Professor, Bioscience

Antonio Adamo

Professor Adamo's research interests focus on the use of human embryonic stem cells (hESCs) and patient-derived induced pluripotent stem cells (iPSCs) to model the onset and progression of human disorders linked to copy number variations “in a dish.” His team developed the largest cohort of Klinefelter syndrome (karyotype 47,XXY) and high-grade X aneuploid iPSCs (karyotype 48,XXXY and 49,XXXXY) that he uses to study the molecular dysregulations associated with X chromosome aneuploidy during the earliest stages of human development. Professor Adamo’s team combines reprogramming, organoid derivation, and genome editing techniques with a multi-omics approach to identify the transcriptional and epigenetic signatures underlying human diseases.

Desired Project Deliverables

​The selected candidate will use human stem cells and terminally differentiated glucose sensitive cell types and will acquire skills in molecular biology techniques including Chromatin Immuno-precipitation (ChIP), quantitative real-time PCR (Q-PCR) and next generation sequencing (NGS).​