Spatio temporal analysis of expression of genes controlling assymetric stem cell division and tissue patterning in plants


Project Description

BIRDs nuclear factors have been described to regulate root growth through association with the transcription factors SCARECROW and SHORTROOT, however their function in other organs remain to be elucidated. Here we propose to dissect network function in lateral roots and leaves. We will determine their physical associations spatially and during different developmental stages. We will also assess whether their target are regulated similarly.Objectives: In this project we aim to dissect how BIRD proteins regulate leave tissue patterning and map their localization in different mutant backgrounds. In addition, we will dissect binding sites in different target genes and alter specific binding by site directed mutagenesis. Technologies: confocal imaging microscopy, site directed mutagenesis, promoter activities using dual luciferase, plant phenotyping, cloning using gateway technology ​
Program - Plant Science
Division - Biological and Environmental Sciences and Engineering
Center Affiliation - Center for Desert Agriculture
Field of Study - ​Plant Biology​

About the

Ikram Blilou

Ikram Blilou

Desired Project Deliverables

​Map the expression of the genes at different developmental stages and dissect thein binding motifs